Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485/491 (S-G-SP-V-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q13131 P54646
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
AMPK1/AMPK2 (Ab-485/491) antibody detects endogenous levels of total AMPK1/ AMPK2 protein.
References:
Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPK1/AMPK2 (Ab-485/491) antibody (E021130).