Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCμ around the phosphorylation site of serine 910 (R-V-SP-I-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15139
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
PKD/PKCμ (Ab-910) antibody detects endogenous levels of total PKD/PKCμ protein.
References:
Matthews SA, et al. J Biol Chem 1999 Sep; 274(37): 26543-26549
Brandlin I, et al. J Biol Chem 2002 Feb; 277(8): 6490-6496
Storz P, et al. Mol Pharmacol 2004 Oct; 66(4): 870-879
Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PKD/PKCμ (Ab-910) antibody (E021128, Lane 1 and 2) and PKD/PKCμ (phospho-Ser910) antibody (E011096, Lane 3 and 4).