Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCμ around the phosphorylation site of serine 738 (E-K-SP-F-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15139
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
PKD/PKCμ (Ab-738) antibody detects endogenous levels of total PKD/PKCμ protein.
References:
Storz P, et al. Mol Cell Biol. 2004 Apr; 24(7): 2614-2626.
Storz P, et al. Mol Cell Biol. 2005 Oct; 25(19): 8520-8530.
Zhang W, et al. J Biol Chem 2005 May 13; 280(19): 19036-19044
Western blot analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10min), using PKD/PKCμ (Ab-738) antibody (E021126, Lane 1 and 2) and PKD/PKCμ (phospho- Ser738) antibody (E011078, Lane 3 and 4).