Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MyoD around the phosphorylation site of serine 200 (A-S-SP-P-R) .
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15172
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
MyoD (Ab-200) antibody detects endogenous levels of total MyoD protein.
References:
Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776
Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24(4): 1809-1821.
Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19(4): 3167-3176.
Western blot analysis of extract from HeLa cells, using MyoD (Ab-200) antibody (E021124).