Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P12931
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Src (Ab-418) antibody detects endogenous levels of total Src protein.
References:
Dufilho M, et al. (2005) J Biol Chem. 280(43):35999-36006.
Sanders MA, et al. (2005) J Biol Chem. 280(25): 23516-23522.
Murugappan S, et al. (2005) Blood. 106(2):550-557.
Gu JJ, et al. (2005) Blood. 105(8): 3270-3277.
Zhou S, et al. (2004) J Biol Chem.279 (52): 54463-54469.
Western blot analysis of extracts from COLO205 cells using Src (Ab-418) antibody (E021115, Lane 1 and 2) and Src (phospho-Tyr418) antibody (E011091, Lane 3 and 4).