• Catalog Number:

  E021114-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 317 (S-S-SP-Q-P)

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Rat

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100

• Specificity Sensitivity:

  Chk1 (Ab-317) antibody detects endogenous levels of total Chk1 protein.

• References:

  Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 (Ab-317) antibody (E021114).

MCF7 | COLO Western blot analysis of the extracts using Chk1 (Ab-317) antibody (E021114).