Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ICAM-1 around the phosphorylation site of tyrosine 512 (K-K-YP-R-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05362
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
ICAM-1 (Ab-512) antibody detects endogenous levels of total ICAM-1 protein.
References:
Greenwood J, et al. (2003) J Immunol; 171(4):2099-2108.
Zhou Z, et al. (2005) Eur J Pharmacol; 513(1-2):1-8.
Chen YH, et al. (2001) J Cell Biochem; 82(3):512-521
Western blot analysis of extracts from HUVEC cells, using ICAM-1(Ab-512) Antibody (E021106, Lane 1, 2 and 3) and ICAM-1(Phospho-Tyr512) Antibody (E011083, Lane 4, 5 and 6).