Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human β-Catenin around the phosphorylation site of threonine 41/serine 45 (A-T-TP-T-A-P-SP-L-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35222
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
β-Catenin (Ab-41/45) antibody detects endogenous levels of total β-Catenin protein.
References:
Provost E, et al. (2003)J Biol Chem 22; 278(34): 31781-9.
Sakanaka C , et al. (2003)J Biochem (Tokyo); 132(5): 697-703.
Amit S, et al. (2002) Genes Dev; 16(9): 1066-76.
Liu C, et al. (2002) Cell; 108(6): 837-47.
Provost E, et al. (2005) Oncogene; 24(16): 2667-76.
Western blot analysis of extracts from SW626 cell using β-Catenin (Ab-41/45) antibody (E021103, Lane 1 and 2) and β-Catenin (phospho-Thr41/Ser45) antibody (E011116, Lane 3 and 4).