Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Tau around the phosphorylation site of threonine 231 (V-R-TP-P-P)..
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
Tau (Ab-231) antibody detects endogenous levels of total Tau protein.
References:
Yen S.H.et.al. (1997) Neurochem. 69:1709-1719.
Beausoleil S.A.et.al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135.
Wintjens R.et.al. (2001) Biol. Chem. 276:25150-25156.
Babu J.R.et.al.(2005) Neurochem. 94:192-203.
Goedert M., Spillantini M.G. (2000) Biochim. Biophys. Acta 1502:110-121.
Western blot analysis of extract from mouse brain tissue using Tau (Ab-231) Antibody (E021099, Lane 1 and 2) and Tau (phospho- Thr231) antibody (E011110, Lane 3 and 4).
Immunohistochemical analysis of paraffin- embedded rat hippocampal region tissue from a model with Alzheimer’s Disease using Tau (Ab-231) Antibody (E021099).