Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Tau around the phosphorylation site of serine 396 (Y-K-SP-P-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Tau (Ab-396) antibody detects endogenous levels of total Tau protein.
References:
Puig B, et al. (2005) Acta Neuropathol (Berl). 110(3):261-268.
DeGiorgis JA, et al. (2005) J Proteome Res. 4(2): 306-315.
Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.
Western blot analysis of extract from mouse brain tissue using Tau (Ab-396) antibody (E021093, Lane 1 and 2) and Tau (phospho-Ser396) antibody (E011102, Lane 3 and 4).