• Catalog Number:

  E021090-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 46 (M-L-SP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  p53 (Ab-46) antibody detects endogenous levels of total p53 protein.

• References:

  Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442. Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959. Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.

Peptide - + Western blot analysis of extracts from 293 cells treated with UV, using p53 (Ab-46) antibody (E021090).