Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human P53 around the phosphorylation site of serine 9 (D-P-SP-V-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P04637
Applications:
WB:1:500~1000
Specificity Sensitivity:
P53 (Ab-9) Antibody detects endogenous levels of total P53 protein.
References:
Ito, A. et al. (2001) EMBO J. 20, 1331-1340.
Sakaguchi, K. et al. (1998) Genes Dev. 12, 2831-2841.
Solomon, J.M. et al. (2006) Mol. Cell. Biol. 26, 28-38.
Western blot analysis of extract from HT-29 cells, using P53 (Ab-9) Antibody (E021084, Lane 1 and 2).