Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1175 (K-D-YP-I-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P35968
Applications:
IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
VEGFR2 (Ab-1175) antibody detects endogenous levels of total VEGFR2 protein.
References:
Lamalice L, et al. (2004). Oncogene.23(2): 434-445.
Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Ab-1175) antibody (E021077).
Immunofluorescence staining of methanol-fixed MCF7 cells using VEGFR2 (Ab-1175) antibody (E021077, Red).