• Catalog Number:

  E021060-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  BCL-2 (Ab-70) antibody detects endogenous levels of total BCL-2 protein.

• References:

  Muller IM, et al. (2005) Mol Pharmacol. Jin Z, Gao F,et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19. Kumar Biswas S,et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34. Huang ST,et al. (2002)FASEB J; 16(8): 825-32. Yamamoto K,et al. (1999) Mol Cell Biol; 19(12): 8469-78.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70) antibody (E021060).

Peptide + - Western blot analysis of extracts from MCF7 cells using BCL-2 (Ab-70) antibody (E021060).