Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P60484
Applications:
WB: 1:500~1:1000 IHC:1:50~100
Specificity Sensitivity:
PTEN (Ab-380/382/383) antibody detects endogenous levels of total PTEN protein.
References:
Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.
Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.
Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (Ab-380/ 382/383) antibody (E021056).
Western blot analysis of extracts from HT29 cells using PTEN (Ab-380/382/ 383) antibody (E021056).