Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P40763
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
STAT3 (Ab-705) antibody detects endogenous levels of total STAT3 protein.
References:
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804.
Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115.
Garcia R, et al. (1998) J Biomed Sci. 5: 79-85.
Bromberg J F, et al. (1999) Cell. 98: 295-303.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Ab-705) antibody (E021045).
Western blot analysis of extracts from K562 cells using STAT3 (Ab-705) antibody (E021045).