Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15976
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
GATA-1 (Ab-142) antibody detects endogenous levels of total GATA-1 protein.
References:
Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672.
Trainor C D, et al. (1990) Nature. 343: 92-96.
Nichols K E, et al. (2000) Nat Genet. 24: 266-270.
Freson K, et al. (2001) Blood. 98: 85-92.
Mehaffey M G, et al. (2001) Blood. 98: 2681-2688.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (Ab-142) antibody (E021041).
Western blot analysis of extracts from K562 cells using GATA-1 (Ab-142) antibody (E021041).