Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 389 (P-R-SP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19419
Applications:
IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
Elk-1(Ab-389) antibody detects endogenous levels of total Elk-1 protein.
References:
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73:381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824.
Hill C S, et al. (1995) Cell. 80:199-211.
Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Elk-1 (Ab-389) antibody (E021037).
Immunofluorescence staining of methanol-fixed HeLa
cells using Elk-1 (Ab-389) antibody (E021037, Red).