Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of threonine 73 or 55 (T-P-TP-R-F).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15336
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
ATF-2 (Ab-73 or 55) antibody detects endogenous levels of total ATF-2 protein.
References:
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (Ab-73 or 55) antibody (E021032).
Western blot analysis of extracts from LOVO cells using ATF-2 (Ab-73 or 55) antibody (E021032).