Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of threonine 71 or 53 (T-P-TP-P-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15336
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
ATF-2(Ab-71or 53) antibody detects endogenous levels of total ATF-2 protein.
References:
Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.
Waetzig G H, et al. (2002) J Immunol. 168(10): 5342-5351.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using ATF-2 (Ab-71or 53) antibody (E021031).
Western blot analysis of extracts from HeLa cells using ATF-2 (Ab-71or 53) antibody (E021031).