Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 91 (T-T-TP-P-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05412
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
c-Jun (Ab-91) antibody detects endogenous levels of total c-Jun protein.
References:
Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Ab-91) antibody (E021021).
Western blot analysis of extracts from HeLa cells using c-Jun (Ab-91) antibody (E021021).