Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 468 (L-A-SP-V-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q04206
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
NF-κB p65 (Ab-468) antibody detects endogenous levels of total NF-κB p65 protein.
References:
Buss H, et al. (2004) J Biol Chem
Schmitz ML, et al. (2004) Blood
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p65 (Ab-468) antibody (E021013).
Western blot analysis of extracts using NF-κB p65 (Ab-468) antibody (E021013).