Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GSK3α around the phosphorylation site of serine 21 (T-S-SP-F-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49840
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
GSK3α (Ab-21) Antibody detects endogenous levels of total GSK3α protein.
References:
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Cross D A, et al. (1995) Nature. 378: 785-789.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3α (Ab-21) antibody (E021007).
Western blot analysis of extracts from ovary cancer cells using GSK3α (Ab-21) antibody (E021007)