Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 383 (T-L-SP-P-I).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19419
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
Elk-1(Ab-383) antibody detects endogenous levels of total Elk-1 protein.
References:
Xing J, et al. (1996) Science. 273(5277): 959-963.
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73: 381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.
Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.
Whitmarsh A J, et al. (1995) Science. 269:403-407.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Elk-1 (Ab-383) Antibody (E021004).
Western blot analysis of extracts from HeLa cells using Elk-1(Ab-383) Antibody (E021004).