Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05412
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
c-Jun ( Ab-63) antibody detects endogenous levels of c-Jun protein around serine 63.
References:
Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
Sevilla A, et al. (2004). Oncogene.23 (55): 8950-8958.
Li L, et al. (2004). J Biol Chem.279 (6): 4058-4065.
Hurd C, et al. (2002). Oncogene.21 (14): 2154-2160.
Peptide - +
Western blot analysis of extract from NIH/3T3 cells treated with UV, using c-Jun( Ab-63) antibody (E021001).