• Catalog Number:

  E012093-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 612 (P-M-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35569

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  IRS-1 (Phospho-Ser612) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 612.

• References:

  Andreozzi F, et al. (2004) Endocrinology 145, 2845-57. Esposito DL, Li Y, Cama A, Quon MJ (2001) Endocrinology 142, 2833-40. Rikova K, et al. (2007) Cell 131, 1190-203. Sun, X.J. et al. (1991) Nature 352, 73-77. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (Phospho-Ser612) Antibody (E012093).