• Catalog Number:

  E012085-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423 (E-F-SP-D- SP –E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13547

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  HDAC1 (Phospho-Ser421/423) antibody detects endogenous levels of HDAC1 only when phosphorylated at serine 421/423.

• References:

  Dephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7. Han G, et al. (2008) Proteomics 8, 1346-61. Molina H, et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204. Olsen JV, et al. (2006) Cell 127, 635-48. Pflum MK, Tong JK, et al. (2001) J Biol Chem 276, 47733-41. Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 (Phospho-Ser421/423) antibody (E012085).

Western blot analysis of extracts from HELA cells. Left:Using HDAC1 (Phospho-Ser421/423) antibody (E012085). Right: Using the same antibody preincubated with synthesized peptide.