• Catalog Number:

  E012082-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 659 (V-D-YP-V-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13480

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  Gab1 (Phospho-Tyr659) antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 659.

• References:

  Hoffmann KM, Tapia JA, Jensen RT (2006) Cell Signal 18, 942-54. Montagner A, et al. (2005) J Biol Chem 280, 5350-60. Cunnick JM, Mei L, Doupnik CA, Wu J (2001) J Biol Chem 276, 24380-7. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564. Weidner, K.M. et al. (1996) Nature 384, 173-176. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using Gab1 (Phospho-Tyr659) Antibody (E012082).