Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FGFR1 around the phosphorylation site of RMPVAPYWTSPEK tyrosine 154 (A-P-YP-W-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P11362
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity Sensitivity:
FGFR1 (Phospho-Tyr154) antibody detects endogenous levels of FGFR1 only when phosphorylated at tyrosine 154.
References:
DiGabriele A.D., et al. (1998) Nature 393:812-817.
Cross M.J., et al. Mol. (2002) Biol. Cell 13:2881-2893.
Hinsby AM, Olsen JV, Bennett KL, Mann M (2003) Mol Cell Proteomics 2, 29-36.
Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using FGFR1 (Phospho-Tyr154) Antibody (E012077).