Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207 (H-A-YP-A-Q).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P46109
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity Sensitivity:
CrkL (Phospho-Tyr207) antibody detects endogenous levels of CrkL only when phosphorylated at tyrosine 207.
References:
Rush J., et al. (2005) Nat. Biotechnol. 23:94-101.
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.
Wolf-Yadlin A, et al. (2006) Mol Syst Biol 2, 54.
Satter, M. and Salgia, R. (1998) Leukemia 12, 637-644.
Feller, S. M. et al. (1998) J. Cell. Physiol. 177, 535-552.
Kiyokawa, E. et al. (1997) Crit. Rev. Oncog. 8, 329-342.
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CrkL (Phospho-Try207) Antibody (E012071).