• Catalog Number:

  E012067-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of threonine 387 (C-G-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  CHK2 (Phospho-Thr387) antibody detects endogenous levels of CHK2 only when phosphorylated at serine 317.

• References:

  Gabant G, et al. (2008) J Mol Biol 380, 489-503. Gupta SK, et al. (2007) J Biol Chem 282, 29431-40. Oliva-Trastoy M, et al. (2007) Oncogene 26, 1449-58. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK2 (Phospho-Thr387) Antibody (E012067).

Western blot analysis of extracts from Jurkat cells using CHK2 (Phospho-Thr387) Antibody (E012067).