• Catalog Number:

  E012059-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q99683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  ASK1 (phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.

• References:

  Ichijo, H. et al. (1997) Science 275, 90-94. Wang, X.S. et al. (1996) J. Biol. Chem. 271, 31607-31611. Matsuzawa, A. and Ichijo, H. (2001) J. Biochem. (Tokyo) 130, 1-8. Tobiume, K. et al. (2001) EMBO Rep. 2, 222-228. Nishitoh, H. et al. (2002) Genes Dev. 16, 1345-1355. Zhang, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8511-8515.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using ASK1 (Phospho-Ser966) Antibody (E012059).