• Catalog Number:

  E012058-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-SP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q99683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  ASK1 (phospho-Ser83) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 83.

• References:

  Mochizuki T, et al. (2006) Oncogene 25, 3699-707. Song JJ, Lee YJ (2005) J Biol Chem 280, 26845-55. Ichijo, H. et al. (1997) Science 275, 90-94. Wang, X.S. et al. (1996) J. Biol. Chem. 271, 31607-31611. Matsuzawa, A. and Ichijo, H. (2001) J. Biochem. (Tokyo) 130, 1-8.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using ASK1 (Phospho-Ser83) Antibody (E012058).