• Catalog Number:

  E012055-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ALK around the phosphorylation site of tyrosine 1586 (Y-G-YP-Q-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q9UM73

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  ALK (phospho-Tyr1586) antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1586.

• References:

  Rikova K, et al. (2007) Cell 131, 1190-203. Stoica, G.E. et al. (2001) J. Biol. Chem. 276, 16772-16779. Iwahara, T. et al. (1997) Oncogene 14, 439-449. Morris, S.W. et al. (1997) Oncogene 14, 2175-2188. Morris, S.W. et al. (1994) Science 263, 1281-1284. Bai, R.Y. et al. (1998) Mol. Cell Biol. 18, 6951-6961.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using ALK (Phospho-Tyr1586) Antibody (E012055).