Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Abl1 around the phosphorylation site of tyrosine 204 (K-L-YP-V-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P00519
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity Sensitivity:
Abl1 (phospho-Tyr204) antibody detects endogenous levels of Abl1 only when phosphorylated at tyrosine 204.
References:
Wang J.Y. et al. (2000) Oncogene 19, 5643-5650.
Danial N.N. et al. (2000) Oncogene 19, 2523-2531.
Pluk H. et al. (2002) Cell 108, 247-259.
Rikova K, et al. (2007) Cell 131, 1190-203.
Goss VL, et al. (2006) Blood 107, 4888-97.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Abl1 (Phospho-Tyr204) Antibody (E012053).