• Catalog Number:

  E012022-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of Threonine 3 (A-R-TP-K-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P68431

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  Histone H3.1 (phospho-Thr3) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Threonine 3.

• References:

  Bonenfant D, et al. (2007) Mol Cell Proteomics 6, 1917-32 Dai J, Sultan S, Taylor SS, Higgins JM (2005) Genes Dev 19, 472-88 Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45. Cheung, P. et al. (2000) Cell 103, 263-271. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human malignant lymphoma tissue using Histone H3.1 (phospho-Thr3) antibody (E012022).

Western blot analysis of extracts from HuvEc cells treated with Serum. Left: Using Histone H3.1 (phospho-Thr3) antibody (E012022). Right: Using the same antibody preincubated with synthesized peptide.