• Catalog Number:

  E012017-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204 (F-L-TP-E -YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P27361

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  ERK1/2 (phospho-Thr202/Tyr204) antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202/Tyr204.

• References:

  Marais, R. et al. (1993) Cell 73, 381-93. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. Pagès G (2007) J Cell Physiol 213, 454-63 Basbous J, et al. (2007) Mol Cell Biol 27, 3936-50

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ERK1/2 (phospho- Thr202/Tyr204) (E012017).

Western blot analysis of extracts from Jurkat cells treated with PMA. Left: Using ERK1/2 (phospho- Thr202/Tyr204) antibody (E012017). Right: Using the same antibody preincubated with synthesized peptide.