• Catalog Number:

  E012004-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPKbeta1 around the phosphorylation site of serine 182 (S-S-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9Y478

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  AMPKbeta1 (phospho-Ser182) antibody detects endogenous levels of AMPKbeta1 only when phosphorylated at serine 182.

• References:

  Warden, S.M. et al. (2001) Biochem. J. 354, 275-283. Yin W, Mu J, Birnbaum MJ (2003) J Biol Chem 278, 43074-80 Hardie, D.G. (2004) J. Cell Sci. 117, 5479-5487. Shaw, R. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335. Watt MJ, et al. (2006) Am J Physiol Endocrinol Metab 290, E500-8

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AMPKbeta1 (phospho-Ser182) (E012004).

Western blot analysis of extracts from Jurkat cells. Left: Using AMPKbeta1 (phospho-Ser182) antibody (E012004). Right: Using the same antibody preincubated with synthesized peptide.