• Catalog Number:

  E012001-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 65 (R-N-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13541

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  4E-BP1 (phospho-Ser65) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at serine 65.

• References:

  Pause A. et al. (1994) Nature 371, 762-767. Brunn G.J. et al. (1997) Science 277, 99-101. Gingras AC, et al. (2001) Genes Dev 15, 2852-64. Lal L, et al. (2005) Blood 105, 1669-77. Molina H, et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (phospho-Ser65) antibody (E012001).