• Catalog Number:

  E011550-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166 (A-I-SP-E-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q00987

• Applications:

  WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  MDM2 (phospho-Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Serine 166.

• References:

  Haupt, Y. et al. (1997) Nature 387, 296-299. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (phospho-Ser166) Antibody (E011550).

Peptide + - Western blot analysis of extracts from HT29 cell using MDM2 (phospho-Ser166) Antibody (E011550).