• Catalog Number:

  E011545-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of Tyr576/Tyr577 (S-T-YP-YP-K-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  FAK (phospho-Tyr576/Tyr577) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr576/Tyr577.

• References:

  Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613 Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.

Immunofluorescence staining of methanol-fixed HeLa cells using FAK (phospho-Tyr576/Tyr577) Antibody (E011545, Red).