• Catalog Number:

  E011541-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine395 (L-L-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P24864

• Applications:

  IHC: 1:50~100

• Specificity Sensitivity:

  Cyclin E1 (phospho-Thr395) Antibody detects endogenous levels of Cyclin E1only when phosphorylated at threonine395.

• References:

  Lauper, N. et al. (1998) Oncogene 17, 2637-2643. Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193. Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin E1 (phospho-Thr395) Antibody (E011541).