Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
Paxillin around the phosphorylation site of Tyr88 (P-V-YP-G-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P49023
Applications:
IF:1:100~1:200
Specificity Sensitivity:
Paxillin (Phospho-Tyr88) Antibody detects endogenous levels of Paxillin only when
phosphorylated at Tyr88.
References:
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
Immunofluorescence staining of methanol-fixed HeLa cells using Paxillin (phospho-Tyr88) Antibody (E011538, Red).