Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
MARCKS around the phosphorylation site of Serine 170 (G-F-SP-F-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P29966
Applications:
IHC: 1:50~100
Specificity Sensitivity:
MARCKS (phospho-Ser170) Antibody detects endogenous levels of MARCKS only when
phosphorylated at threonine 170.
References:
Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479.
Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823.
Hartwig, J. H. et al. (1992) Nature 356, 618-622.
Thelen, M. et al. (1991) Nature 351, 320-322.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (phospho-Ser170) Antibody (E011535).