Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
IKKa/Ikkb around the phosphorylation site of Serine 180/181 (C-T-SP-F-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human ,Mouse
SwissProtNo:
O15111 O14920
Applications:
IHC: 1:50~100
Specificity Sensitivity:
IKKα/Ikkβ (phospho-Ser180/181) Antibody detects endogenous levels of IKKa/Ikkb only
when phosphorylated at Serine 180/181.
References:
Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
Chen, Z.J. et al. (1996) Cell 84, 853-62.
Delhase, M. et al. (1999) Science 284, 309-13.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IKKα/Ikkβ (phospho-Ser180/181) Antibody (E011532).