Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human
PLC-γ2 around the phosphorylation site of tyrosine 1217 (F-L-YP-D-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16885
Applications:
IHC: 1:50~100
Specificity Sensitivity:
PLC-γ2 (phospho-Tyr1217) Antibody detects endogenous levels of PLC-γ2 onlywhen
phosphorylated at tyrosine 1217.
References:
Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Margolis, B. et al. (1989) Cell 57, 1101-1107.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-γ2 (phospho-Tyr1217) Antibody (E011524).