• Catalog Number:

  E011523-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLC-gamma1 around the phosphorylation site of tyrosine 771 (P-D-YP-G-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse ,Rat

• SwissProtNo:

  P19174

• Applications:

  IF:1:100~1:200

• Specificity Sensitivity:

  PLC-γ1 (phospho-Tyr771) Antibody detects endogenous levels of PLC-gamma1 only when phosphorylated at tyrosine 771.

• References:

  Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.

Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ1 (phospho-Tyr771) Antibody (E011523, Red).