Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human AuroraA around the phosphorylation site of threonine 288 (R-T-TP-L-M).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
O14965
Applications:
IHC: 1:50~100
Specificity Sensitivity:
AuroraA (phospho-Thr288) Antibody detects endogenous levels of Aurora A only when
phosphorylated at threonine 288.
References:
Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
Zhou, H. et al. (1998) Nat Genet 20, 189-93.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AuroraA (phospho-Thr288) Antibody (E011519).