• Catalog Number:

  E011517-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of serine 246/259/220 (T-A-SP- E-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P56524 Q9UQL6 Q9UKV0

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100

• Specificity Sensitivity:

  HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody detects endogenous levels of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.

• References:

  Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Vigushin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

Western blot analysis of extracts from 3T3 cell using HDAC4/ HDAC5/HDAC9 (Ab-246/259/220) Antibody (E021517, Lane 1 and 2) and HDAC4/ HDAC5/HDAC9 (phospho-Ser246/ 259/ 220) Antibody (E011517, Lane 3 and 4).

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/ HDAC9 (phospho-Ser246/259/220) Antibody (E011517).