• Catalog Number:

  E011514-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human eIF4G around the phosphorylation site of serine 1232 (P-V-SP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC:1:50~1:100

• Specificity Sensitivity:

  eIF4G (phospho-Ser1232) Antibody detects endogenous levels of eIF4G only when phosphorylated at serine 1232.

• References:

  De Gregorio, E. et al. (1998) RNA 4, 828-836. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136. Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho-Ser1232) antibody (E011514).

Western blot analysis of extracts from 293 cell using eIF4G (Ab-1232) Antibody (E021514, Lane 1 and 2) and eIF4G (phospho-Ser1232) antibody (E011514, Lane 3 and 4).